Research Assistant/Associate - Spatial -Omics & Molecular Biology

Research Assistant/Associate

• Spatial
• Omics & Molecular Biology BIDMC
• Harvard Medical School
• Broad Institute of MIT and Harvard Boston, MA Job Details Full-time $45,000
• $80,000 a year 14 hours ago Benefits Health savings account Health insurance Dental insurance 401(k) Flexible spending account Paid time off Parental leave Vision insurance 401(k) matching Life insurance Retirement plan Qualifications Laboratory procedures Teamwork Microsoft Excel GLP Specimen collection / processing Scientific research Bachelor's degree Pipetting Full Job Description The Spatial Technologies Unit (STU / www.

spatialtechnology.org) , Beth Israel Deaconess Medical Center / Harvard Medical School Initiative for RNA Medicine / Harvard Cancer Center, is seeking a Research Assistant / Associate

• Spatial Omics & Molecular Biology (RA) The RA will have the ability to train and work on cutting edge single cell and spatial
• omics technologies, next generation sequencing assays, and histology in an exceptional environment.

No prior experience in single cell/spatial assays required. Wet lab experience and training in good laboratory practices are considered essential . Experience in working with tissue (cryostat/microtome), as well as library preparations (NGS) are considered as desired. Spatial Technologies, including Spatial Transcriptomics and Proteomics, were deemed " Method of the Year 2020 " by Nature Methods Journal and are currently revolutionizing biomedical research. The Spatial Technologies Unit is a reference center for the provision and advancement of single cell and spatial tissue profiling, and a focal point for Spatial Technologies research and development in the Commonwealth of Massachusetts and across the United States. It hosts a comprehensive collection of leading spatial and single cell technologies from10x Genomics (Visium, Xenium, Connect, X), Bruker/NanoString (GeoMx), Akoya (PhenoImager, PhenoCycler Fusion), Vizgen (MERSCOPE), Singular Genomics G4X, and Illumina Spatial Technology. Its unparalleled technological infrastructure, its location within the Harvard Medical School Ecosystem, and its translational role in a leading medical center provides exceptional opportunities for cutting-edge research and applications. The STU provides exceptional opportunities for growth and the ability to become a leader in the exciting field of single cell and spatial tissue profiling. For more information on the STU, abilities, and mission, please visit http://www.spatialtechnology.org.

Job Description:

You will have an exceptional opportunity to join our highly-skilled team as a Research Assistant in an exceptional environment for scientific growth. In this role, the successful candidate will be responsible for applying modern molecular and histology techniques, Next Generation Sequencing (NGS) and automated single cell technology under the guidance of Senior Scientists, to support leading research projects related to Spatial Technologies such as spatial transcriptomics, highly multiplexed single cell and Super-Resolution imaging.

Essential Responsibilities:

Performs routine and non-routine laboratory tests which may include next generation sequencing, in situ hybridization, single cell assays, and spatial tissue profiling assays; synthesizes and interprets results. Sections paraffin embedded or frozen tissue using a microtome or cryostat, respectively May operate and maintain moderate to complex lab equipment. Manage the administrative tasks of the lab including requisition, inventory maintenance, and consultation scheduling. Oversee sample archives and performs routine QC for different samples types; Oversee and coordinate instrument maintenance, updates and repairs; Assist in developing and update SOPs and implementing new protocols and procedures. Using analytical abilities and sound judgment, makes decisions regarding the reliability and accuracy of results, repeating procedures when necessary. May assist with planning specific research procedures and coordinating schedules of tests and procedures May prepare written and/or verbal reports on status of research and the technical procedures used, including statistical and graphic summaries of findings and possible conclusions.

Required Qualifications:

Bachelor's degree in Life Sciences or related scientific field required Basic wet-lab experience: pipette handling, buffer preparation, good laboratory practices. 0-4 Years Related Work Experience Required. Experience with computer systems required, including web based applications and some Microsoft Office applications which may include Outlook, Word, Excel, PowerPoint or Access. Useful Experience (not required but will be evaluated) Reagent preparation (solution, buffer and medium), DNA/RNA extraction and purification from tissues or cells, PCR/qPCR and molecular cloning. Working with RNA Tissue sectioning, paraffin-embedded / frozen Immunohistochemistry on animal or human samples, cell cytometry and sorting.

Competencies:

Decision Making:

Ability to make decisions that are based on specific instructions, standard practices and established procedures which generally require little or no supervision.

Problem Solving:

Ability to address problems that are routine, somewhat repetitive and generally solved by following clear directions and procedures and by identifying opportunities for process improvements.

Independence of Action:

Ability to follow general instructions and procedures as provided. Work is monitored by supervisor/manager.

Written Communications:

Ability to read, and write in English in order to understand basic safety instructions and take direction from supervisors; communicate effectively with patients, families and other medical center staff; and respond to basic questions.

Oral Communications:

Ability to understand spoken English in order to follow basic safety instructions and take direction from supervisors; communicate effectively in basic English with patients, families and medical center staff in response to routine questions.

Knowledge:

Ability to demonstrate basic knowledge of fundamental concepts, practices and procedures with the ability to use them in routine situations. Specialized knowledge not required.

Team Work:

Ability to interact respectfully with other employees, professional staff and/or external contacts to offer ideas, identify issues, obtain information or deliver services.

Customer Service:

Ability to demonstrate a positive attitude and respond to requests in a t As a health care organization, we have a responsibility to do everything in our power to care for and protect our patients, our colleagues and our communities. Beth Israel Lahey Health requires that all staff be vaccinated against influenza (flu) and

COVID-19

as a condition of employment. Learn more about this requirement. More than 35,000 people working together. Nurses, doctors, technicians, therapists, researchers, teachers and more, making a difference in patients' lives. Your skill and compassion can make us even stronger.

Equal Opportunity Employer/Veterans/Disabled Job Type:

Full-time Pay:

$45,000.00

• $80,000.

00 per year

Benefits:

401(k) 401(k) matching Dental insurance Flexible spending account Health insurance Health savings account Life insurance Paid time off Parental leave Retirement plan Vision insurance

Experience:

NGS library preparation: 1 year (Preferred) tissue sectioning: 1 year (Preferred) good laboratory practices: 1 year (Preferred) Laboratory procedures: 1 year (Preferred)

Work Location:

In person