I - ADRD

Job ID:

req4603\n

Employee Type:

nonexempt full-time\n

Division:

Applied & Development Research Program\n

Facility:

Frederick:

Ft Detrick\n

Location:

PO Box B, Frederick, MD 21702 USA\nThe Frederick National Laboratory is operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases.\nAccountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way.\n

PROGRAM DESCRIPTION

\nThe In Vivo Evaluation Group, Biological Testing Branch (BTB) of the Developmental Therapeutics Program (DTP), Division of Cancer Treatment and Diagnosis (DCTD) is establishing both in vitro and in vivo tumor lines from patient biopsies from DCTD sponsored clinical trials with the goal of developing models for NCI's Patient Derived Models Repository (PDMR). The PDMR is a repository of patient-derived models that include in vivo tumor fragments, in vitro tumor lines (2D and 3D), cancer associated fibroblasts, and DNA/RNA isolates. The goal of the PDMR is to distribute well validated and highly characterized material to researchers worldwide.\n

KEY ROLES/RESPONSIBILITIES

\nAid in the daily maintenance and cryopreservation of the various cultures grown in different medias\nEstablish organoid cultures from patient samples and from tumors isolated from mice\nPrepare cultures for the various validation assays (flow cytometry, cell sorting, Q RT PCR, soft agar clonal assays, RNA/DNA extraction, the STR identity assay, proliferation assays, etc)\nPrepare all required medias, components and reagents used in the culture of organoid cultures\nParticipate in evaluating the effects of different basement membrane extracts, media additives, as well as different protocols on the establishment and growth of both 2D and 3D cell lines\nAid in other routine tasks as needed such as cell photography, PowerPoint presentations, tube/plate labeling, cell deliveries/pick-ups etc\nMaintain precise documentation of each 2D/3D cell line in Excel spreadsheets.\n

BASIC QUALIFICATIONS

\nTo be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:\nPossession of a Bachelor's degree from an accredited college or university according to the Council for Higher Education Accreditation (CHEA), or four (4) years relevant experience in lieu of degree. Foreign degrees must be evaluated for U.S. equivalency\nIn addition to the education requirement, a minimum of two (2) years of progressively responsible experience\nAbility to be flexible to meet changing needs\nOrganizational skills and database management skills\nAbility to work in a fast-paced environment\nAbility to work under Biosafety Level-2 conditions\nAbility to work alone and as part of a team\nAbility to obtain and maintain a security clearance\n

PREFERRED QUALIFICATIONS

\nCandidates with these desired skills will be given preferential consideration:\nKnowledge of sterile eukaryotic tissue culture techniques\nKnowledge of organoid cell culture\n

JOB HAZARDS

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That's why we craft compensation packages that reflect the importance of the work we do for our customers. Employment benefits include competitive compensation, Health and Wellness programs, Income Protection, Paid Leave and Retirement. More details are available 58,200.00 - 83,353.00 USD\nThe posted pay range for this job is a general guideline and not a guarantee of compensation or salary. 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