We are seeking a

• _Research Specialist II_
• to join the lab at Johns Hopkins All Children's Hospital in St.

Petersburg, Florida. This position will work in a lab focused on cancer immunology research investigating the tumor microenvironment and anti-tumor immune responses, including organoid and primary cell culture systems and in vivo preclinical tumor models. The specialist will contribute to immunotherapy and translational research studies within a collaborative and dynamic laboratory environment. The Research Specialist II develops assays and protocols for PI approval and designs experimental approaches for laboratory projects. Performs a range of sophisticated assays. Makes significant contributions to various aspects of laboratory efforts and goals. Responsible for troubleshooting, independent decisions and training/guidance of other laboratory personnel.

• Specific Duties & Responsibilities
• + Conduct assigned laboratory projects.

+ Utilize specialized knowledge to interpret results, e.g. read controls, evaluate results, etc.

+ Work independently and use scientific knowledge and lab experience to make judgments and decisions.

+ Teach/train learners, and new technicians in the laboratory.

+ Guide, direct, and facilitate problem solving for entry-level laboratory members on day-to-day problems with more challenging research assays.

+ Make scientific contributions to protocol development, efficiency, and productivity.

+ Collaborate in the development of new techniques and instruments.

+ Apply published research methods to current experimental work by investigating published protocols and discussing them with PI.

+ Scientifically contribute to the preparation of manuscripts and proposals.

+ Process analyzed data into figures for scientific publications.

+ Order equipment and supplies, interface with vendors.

+ Maintain daily records of experiments.

+ Organize and track laboratory samples.

+ Other duties as assigned.

• Minimum Qualifications
• + Bachelor's Degree in Biology, Chemistry or related field.

+ Two years of laboratory experience performing relevant techniques including experimental techniques used in genomics, sequencing, and cancer biology assays.

+ Additional education may substitute for required experience and additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.

• Preferred Qualifications
• + Master's Degree with related graduate research. + Cell culture. + RNA isolation and quantitative PCR (qPCR). + Common cell biology assays. + Genomics and sequencing. + Immunostaining. + Western blotting. + FACS analysis. + Mouse handling. + Bioinformatics analysis. + GraphPad/Prism/Adobe.
• Technical Skills & Expected Level of Proficiency
• + Assay Development and Validation
• Developing + Data Management and Analysis
• Developing + Experimental Design and Execution
• Developing + Operations Management
• Developing + Protocol Development and Optimization
• Developing + Scientific Writing
• Developing + Stakeholder Engagement
• Developing _The core technical skills listed are most essential; additional technical skills may be required based on specific division or department needs.

_

Classified Title:

Research Specialist II Role/Level/Range:

ACRP/03/MB

Starting Salary Range:

$42,330

• $74,110 Annually ($55,650 targeted; Commensurate w/exp.

) Employee group:

Full Time Schedule:

Mon-Fri, 8.30am-5.00pm

FLSA Status:

Exempt Location:

Florida Department name: All Childrens Personnel area: School of MedicineEqual Opportunity Employer All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.