Banner Position Number:

R10825

Department:

SOM-BioMedical Science - RC5130 Job Description Position Overview The Xu Lab, in the Department of Biomedical Sciences at Marshall University's Joan C. Edwards School of Medicine, is seeking a part-time technician. The primary objective of this position is to construct genetic mutants of Streptococcus pyogenes .

Key Responsibilities Mutant Construction:

Design and generate targeted gene mutations in streptococcal strains.

Molecular Cloning:

Perform PCR, restriction cloning, plasmid purification, and gel electrophoresis.

Bacterial Transformation:

Introduce recombinant DNA into bacteria using electroporation or chemical competence.

Strain Verification:

Validate mutant strains using screening PCR and DNA sequencing data analysis.

Inventory Tracking:

Maintain clean records of primers, plasmids, and newly created glycerol stocks.

Salary Range:

Salary is commensurate with experience and internal equity Education Bachelors Degree in Microbiology, Molecular Biology, Biochemistry, or a related biomedical field

Experience Experience:

Prior hands-on experience with molecular cloning protocols is highly preferred.

Core Skills:

Proficiency in aseptic technique, bacterial culturing, and standard PCR assays.

Attributes:

Strong attention to experimental detail and reliable data documentation habits.

Posting Number:

MR0862

Open Until Filled:

Yes