Job Description Help for Job Description. Opens a new window. The Griffin Lab is seeking a motivated research assistant to support studies on nuclear receptor signaling and innate/adaptive immune responses. The successful candidate will perform primary cell culture, characterize immune cell responses, and analyze target gene expression changes using biochemical, molecular and cellular biology techniques.

Responsibilities:

Isolate, culture, and maintain primary cells. Perform immune stimulation assays. Quantify target gene expression changes via RT-qPCR and assist with RNA-seq and CUT&RUN sample preparation. Maintain cell lines and perform routine molecular biology (RNA/ DNA extraction, cloning, western blot). Maintain detailed lab notebooks and assist with data analysis/ figure preparation. Manage lab inventory, reagent ordering, and equipment maintenance. Assist with IACUC-related animal procedures (if applicable).

Expected Salary:

Commensurate with experience.

Required Qualifications:

A bachelor's degree in an appropriate area of specialization and two years of experience or an equivalent combination of education and experience. Hands-on experience with primary cell culture and aseptic technique. Experience with RT-qPCR and basic molecular biology methods including plasmid transfection. Organizational skills and attention to detail. Drug, background and reference checks required.

Preferred Qualifications:

MS in immunology, cell biology, molecular biology or related field. Experience with flow cytometry. Experience with immune cell isolation and functional assays. Familiarity with RNA-seq sample prep or bioinformatics basics. Prior experience in an academic research setting. The University of Florida is a member of the State University System of Florida and an Equal Opportunity Employer. All qualified applicants will receive consideration for employment based on merit. This position is eligible for Veterans preference. If you are claiming Veterans preference, please upload a copy of your DD 214 Member Copy 4 with your application for consideration. See our Veteran's Preference Page for more specific information.