The Sammarco Laboratory in the Department of Orthopedic Surgery at Mayo Clinic is seeking a highly motivated and intellectually engaged Research Technologist to join a growing research program focused on the cellular and molecular mechanisms of musculoskeletal regeneration. Our laboratory seeks to understand how fundamental cellular processes direct regenerative healing, with the long-term goal of translating these discoveries into therapies that improve musculoskeletal repair and functional recovery. Our research integrates molecular biology, regenerative biology, and advanced imaging to investigate several complementary questions in tissue regeneration. Current areas of investigation include how cellular metabolism, mitochondrial homeostasis and remodeling, and reactive oxygen species (ROS) regulate tissue patterning during regeneration of complex musculoskeletal tissues; the mechanisms by which sensory nerves promote bone regeneration; the role of cellular senescence in regenerative healing; and the molecular pathways that distinguish regenerative repair from fibrosis. Together, these studies aim to identify biological mechanisms that can be leveraged to develop innovative regenerative therapies for patients. The successful candidate will join a collaborative, multidisciplinary laboratory committed to scientific rigor, innovation, and continuous learning. We seek an adaptable, detail-oriented individual who enjoys solving challenging scientific problems, learning new technologies, and contributing to the development and optimization of experimental approaches. This position offers opportunities for professional growth, increasing scientific independence, and authorship on peer-reviewed publications based on individual contributions. Candidates who demonstrate initiative, sound scientific judgment, and an interest in advancing laboratory methodologies will be particularly well suited for this position. Responsibilities The Research Technologist will perform molecular, cellular, imaging, and animal-based experiments in support of laboratory objectives and sponsored research projects while maintaining rigorous experimental records and ensuring high-quality, reproducible data. Primary responsibilities include mammalian cell culture, mouse handling and experimental procedures, tissue processing, histological and immunohistochemical assays, microscopy, image acquisition and quantitative image analysis. The successful candidate will become proficient in these core techniques and perform them independently following appropriate training. Additional responsibilities include performing standard molecular biology techniques (e.g., qPCR, Western blotting, RNA and DNA isolation, reagent preparation, and other molecular assays), data management and basic statistical analyses, preparation of publication-quality figures and manuscripts, and presentation of research findings during laboratory meetings. The technologist will contribute to experimental planning, critically evaluate the scientific literature to implement and refine laboratory methods, troubleshoot experimental assays, and assist in the development of new technologies and experimental workflows. The individual will also play an important role in laboratory operations by coordinating equipment and supplies, maintaining organized laboratory spaces, standardizing workflows, and supporting collaborations within and outside the laboratory. Routine procedures will be performed independently, while more complex studies will be conducted in collaboration with the Principal Investigator and senior laboratory members. Requires bachelor's degree in biology, chemistry, other relevant science, or an associates degree with 6 years of relevant laboratory experience. Must exhibit a solid understanding of lab techniques, equipment, and safety. Must have the ability to organize and carry out laboratory techniques independently and in a team setting. Experience with basic data analysis is required. This position requires demonstrated writing skills.

Preferred Qualifications:

A bachelor's degree in biology, molecular biology, biomedical sciences, or a related field Candidates will demonstrate proficiency in micropipetting, standard molecular biology techniques, laboratory calculations, and safe wet laboratory practices. Previous experience in an academic biomedical research laboratory is strongly preferred, particularly experience with mammalian cell culture, mouse handling, histology, immunohistochemistry, microscopy, quantitative image analysis, or advanced molecular techniques. Experience preparing publication-quality figures and contributing to scientific manuscripts is desirable. The successful candidate will possess exceptional organizational skills, meticulous attention to detail, and excellent written and verbal communication skills. They would be able to manage multiple projects simultaneously, adapt to evolving research priorities, and work effectively both independently and as part of a collaborative, multidisciplinary research team. A demonstrated commitment to scientific rigor, reproducibility, and continuous learning is essential. Applications submitted without a cover letter will not be considered.