Scientist/ Senior Scientist(Contract) Overland San Francisco, CA Job Details Contract $120,000 - $160,000 a year 9 hours ago Benefits Health insurance Qualifications Biology Research design Record keeping Immunological assays Biology research Molecular Biology Immunological laboratory techniques Design of experiments Flow cytometers Assay development Cell Biology Research project design Doctor of Philosophy Scientific protocols Compliance documentation In vivo studies GraphPad Prism Cytometry Data analysis software Laboratory compliance and regulatory documentation Molecular analysis Full Job Description Position Summary We are seeking a highly motivated and hands-on Scientist/Senior Scientist to support our in vivo CAR-T research program. The successful candidate will focus on in vitro assay development, optimization, and characterization of LVV-based CAR-T approaches, with an emphasis on cell-specific targeting, CAR expression, and functional activity.

Duration:

3 months Key Duties and Responsibilities

• Develop and optimize in vitro assays to evaluate LVV-based in vivo CAR-T approaches.
• Perform multicolor flow cytometry to evaluate transduction, CAR expression, cell specificity, T-cell phenotype, and activation.
• Develop and perform cell-based functional assays, including cytotoxicity, proliferation, and cytokine assays.
• Utilize IncuCyte, ELISA/LEGENDplex, and other relevant platforms.
• Perform molecular assays such as qPCR/ddPCR when needed.
• Analyze and interpret data using FlowJo, GraphPad Prism, and Excel.
• Troubleshoot experiments, optimize protocols, and establish reproducible assay workflows.
• Present experimental results and collaborate with scientists across the research team. Requirements
• Ph.D. in Immunology, Cell Biology, Molecular Biology, or a related field.
• 3+ years of hands-on experience in cell therapy, gene therapy, immunology, or related fields.
• Hands-on experience with lentiviral transduction and in vitro characterization of genetically engineered cells.
• Experience with T-cell or immune-cell assays is highly desirable.
• Demonstrated experience in assay development, optimization, and troubleshooting.
• Proficiency in multicolor flow cytometry and cell-based functional assays.
• Proficiency in FlowJo, GraphPad Prism, and Excel.
• Strong experimental design, troubleshooting, data analysis, and communication skills.
• Ability to work independently in a fast-paced research environment.
• Maintain organized, detailed records to support internal documentation and regulatory requirements.

Job Type:

Contract Pay:

$120,000.00 - $160,000.00 per year

Benefits:

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Title and Salary Work Location:

In person