Job Title:

Scientist

•Cell Line Development Location with zip code: Gaithersburg, MD 20878 (Hybrid)

Duration:

Full time

Roles and Responsibilities:

Supports the planning and execution of research experiments in the context of the laboratory's overall research program.

•Plan and execute the generation of stable cell lines for preclinical and clinical material production to advance candidates including monoclonal antibodies, recombinant proteins, and virus like particles. Gene synthesis including oligo synthesis, plasmid design, and construction. 3 Use molecular biology techniques, including RT-PCR and NGS, for genetic characterization of cell lines. 2 Independently execute all steps of the process including plasmid construction, stable pool generation, single cell cloning, and top cloneselection. Supports laboratory staff on research projects and techniques. Draft and verify data for CMC sections of pre-IND and IND documents to ensure successful registration of candidates. Design, perform, and troubleshoot experiments and analyze data for both development and deliverable projects. Maintains laboratory equipment and the training of others in their use. Maintain and support one or more pieces of laboratory instrumentation. Assists in maintaining adequate space and facilities for ongoing and projected research studies. Maintain and support one or more pieces of laboratory instrumentation. Evaluates, analyzes and interprets research data, communicates results and methods in reports, presentations, and manuscripts.

•Identify, propose, and execute process and procedural changes to improve performance, robustness, productivity, and efficiency by reviewing recent literature and new technologies. Coordinates with junior staff to modify research protocols. Develop and track timelines and experimental plans for multiple projects simultaneously and regularly review records of tasks performed by other team members. Presents research at national scientific meetings and symposia. Prepare oral presentations and posters for internal and external groups/conferences. Present results of clinical and experimental research at recognized scientific meetings by lectures and exhibits (poster sessions). Provides interpretation of results, guidance, and scientific support to the management team. Generate, maintain, and review accurate daily records of experiments and results in a laboratory notebook. Prepare and review experimental plans, proposals, reports, SOPs, and production records Mentors junior level scientists. Train staff on the proper usage of equipment.

Deliverables:

Inspect Equipment

•Ad-Hoc Meet with lab members to present updates

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Ad-Hoc Qualifications:

Experience in an industrial setting focused on the development of stable cell lines for vaccine and biotherapeutic candidates

Required Skills:

Extensive hands-on experience with aseptic technique and mammalian cell culture for the purpose of generating clonally derived cell lines to produce monoclonal antibodies or vaccine candidates

•Demonstrated ability to produce and screen hundreds of clonally derived CHO or 293 cell lines for top performers. Expertise in designing and constructing plasmids for gene expression using molecular cloning methods. Hands-on experience with multiple supporting technologies for cell line engineering including cell counting, high-throughput transfection, single-cell cloning/ deposition, flow cytometry, automated liquid handling systems, and automated small-scale bioreactor systems. Ability to independently design experiments and work plans and manage project timelines for multiple complex projects simultaneously. Experience preparing technical reports, SOPs, data presentations, and documents needed for technology transfer and regulatory filing

•Expertise in molecular biology techniques for genetic characterization of cell lines, including RT-PCR and NGS. About our

Company:

•22nd Century Technologies Inc., (TSCTI) is one the fastest growing healthcare staffing companies in the United States. With presence in all 50 States and 6000+ employees nationwide, we have been providing un parallel healthcare and technology staffing services to Public and Private sectors for over 26 years. Started as a technology staffing company in 1997, we have significantly expanded our healthcare practice to both Clinical and Non-Clinical staffing, serving various facilities, healthcare organizations and government agencies. Our ISO certified staffing practices and technology driven staffing procedures

•from recruitment to onboarding, along with an internal pre-vetted resume database of healthcare professionals, enable us to serve our customers with their immediate staffing needs. TSCTI has extensive experience providing Physicians, Nurses, Pharmacists and other Allied Health professionals to various states

•California, Connecticut, District of Columbia, Florida, Michigan, Minnesota, New Jersey, New York, Texas, Vermont, Virginia to name a few, and Our valued relationships with employees as well as commitment to meet and exceed clients' expectations through our exemplary employees proves our competency in today's healthcare staffing industry. Recognized among "Best Company to Work For" by Forbes, 22nd Century Technologies, Inc., consistently exceeds our clients' expectations by focusing on their absolute satisfaction with jobs while keeping our employees motivated. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability or protected veteran status.