Scientist I (Cell Line Development) Orion Group South San Francisco, CA Job Details Temporary | Full-time | Contract $50

• $54 an hour 1 day ago Benefits Health insurance Dental insurance Vision insurance 401(k) matching Qualifications DNA analysis PCR machines Cellular flow assembly Flow cytometry Flow cytometers RNA isolation Analysis skills Bachelor's degree Mammalian cell culture Cytometry qPCR PCR Full Job Description Scientist I $50
• $54/hr with medical benefits available at a cost 1 year initial contract with possibility of extension Onsite in South San Francisco Work shift (days/times)
•  8am
• 4pm (flexible) Orion Project Services and our largest US client are teaming up on an exciting contract opportunity for entry level Scientists!

The Cell Line Development Group in South San Francisco, CA is seeking a Scientist I/II to join a talented and collaborative team developing CHO cell lines for GMP applications. In this laboratory position the candidate will perform hands-on CHO cell line development and characterization studies in a well-supported and dynamic environment to help advance novel biotherapeutics into human clinical studies. The work will encompass molecular, cellular, and bioprocess development studies relating to the generation and characterization of optimized CHO cell lines for production of our cancer pipeline candidates.

Key Responsibilities:

CHO Cell Line Development & Execution:

Lead the end-to-end execution of CHO cell line development workflows for both pipeline and innovation projects. This includes managing post-transfection selective pressures, high-throughput screening, and scaling cultures from 96-well plates to shake flasks.

Monoclonal Cell Line Generation & Banking:

Expertly perform single-cell cloning to generate and establish monoclonal CHO cell lines. Create, expand, and meticulously characterize master and working cell banks.

Genetic & Molecular Characterization:

Engineer and generate complex DNA vector constructs for expressing antibodies and novel protein formats. Perform deep genetic characterization of cell banks by extracting DNA/RNA for analysis via PCR, qPCR/ddPCR, and Next-Generation Sequencing (NGS).

Advanced Instrumentation & Automation:

Skillfully operate and maintain a suite of key laboratory instruments, including ViCell, Octet, flow cytometers, ambr15, HPLC, and automated imagers.

Cross-Functional Collaboration & Communication:

Present scientific updates at cross-functional meetings, collaborating closely with colleagues in purification, analytical, and formulation development to drive projects forward and meet aggressive timelines.

Data Integrity & Documentation:

Maintain meticulous, contemporaneous records of all experimental designs, data, and analysis in electronic lab notebooks (ELNs), ensuring compliance with standards for data integrity and good documentation practices.

Qualifications:

Associate Scientist

• 3 years experience and at least a Bachelor in a scientific field. Scientist I
• 5 years of experience and at least a Bachelor in a scientific field.

Hands-on CHO cell line development and characterization experience Experience with DNA/RNA for analysis via PCR, qPCR/ddPCR is preferred. Flow cytometry.

Job Types:

Full-time, Contract, Temporary Pay:

$50.00

• $54.

00 per hour

Benefits:

401(k) matching Dental insurance Health insurance Vision insurance Application Question(s): Do you have hands-on CHO cell line development and characterization experience?

Education:

Bachelor's (Required)

Experience:

dd

PCR,:

1 year (Preferred) Flow cytometry: 1 year (Required)

PCR:

1 year (Required) Mammalian cell culture: 1 year (Required)

Work Location:

In person