Cryo-Electron Microscopy Senior Scientist in Blue Sky Protein Kinases Position Available In Shelby, Tennessee

Job Description:

We are looking for an experienced cryo – electron microscopy(CryoEM) senior scientist to participate in our exciting studies ofthe human protein kinome. As part of the largest BlueSkyinitiative, “Seeing the Invisible in Protein Kinases” at St. JudeChildren’s Research Hospital, we are expanding our structuralstudies of the human kinome to identify novel kinase-bindingligands. We are looking for experienced CryoEM scientist to aid inthese efforts.

The World’s Brightest Minds Always Innovate – -At St.

Jude Children’s Research Hospital, we know what can be achievedwhen the brightest scientific minds face the fewest barriers.

That’s why we provide world-class facilities. State-of-the-arttechnologies. Extraordinary support. And a collaborative,bench-to-bedside environment where you can see, firsthand, how yourscience translates into survival for the children we serve. Quitesimply, at St. Jude, we encourage you to dream big and stop atnothing when it comes to finding cures and saving children. -The-at St. Jude is at the forefront of scientific discovery,supporting its scientists and investigators in their researchpursuits. The Center offers an entire Single Particle Analysis(SPA) solution and has rapidly progressed into the domain ofCryo-Electron Tomography (CryoET). Recently, the Center acquiredcutting-edge instrumentation, including a Titan Krios G4 electronmicroscope (TEM) featuring a ‘cold-FEG’ electron source, aSelectrisX energy filter, and a Falcon 4i camera.

Moreover, theCenter is supported by St. Jude’s high-performance computingfacility with dedicated GPU and CPU nodes and a larger sharedfilesystem. The research activity will be supported by Blue Skyinitiative. This role will involve working closely as part of amultidisciplinary team including biochemists, NMR spectroscopists,structural biologists, computational chemists, and otherbiophysical specialists.

Ideal Candidate:

Critically evaluate the feasibility of Protein Kinase mediatedcomplexes for CryoEM studies. Experience in CryoEM workflow, structure determination of humanprotein cell signaling complexes. Strong command of CryoEM/ET dataprocessing. Previous experience in working with a multidisciplinary teamsupporting and managing multiple projects simultaneously. At least few years of experience in the vitrification ofbiological samples using semi-automated devices such as VitrobotIII or IV, Leica GP2 plunger. At least three years of experience independently operating anArctica, a Talas, or a Titan Krios electron microscope. Proficiency in SPA or tilt series data collection usingprograms such as EPU, TOMO5, and SerialEM. Hands-on experience with image processing and using one or twoprograms, such as RELION, cryoSPARC, EMAN2, and model analysissoftware, such as COOT and Chimera.

Key Responsibilities Extensive experience in applying CryoEM techniques to determinethe structure of protein complexes. Support the CryoEM projects of the team, by working closelywith other team members. Coordinate with the leaders of the project to design CryoEMstudies with specific goals objectives and experimental plan. Prepare grids for samples provided by researchers across theteam. Implement workflows that will help the researchers with theirCryoEM studies. Provide training to the team members on instrument operationand sample preparation techniques. Independently troubleshoot, solve difficult problems, andpropose solutions related to research project design andexecution Work effectively in a collaborative team environment whereresults are achieved through influence, cooperation, and theincorporation of multiple points of view Perform additional duties as assigned, such as performingFIB-milling. Training can be provided. Stay well-aware about the progress in the field of Humankinases and should present new findings in journal clubs anddepartment seminars. Independently motivated, detail oriented and goodproblem-solving ability Excellent organizational skills, with an ability to embracechange and multi-task in an extremely fast-paced environment Excellent communications skillsMinimum Education and/or

Training:

Bachelor’s degree in relevant scientific area is required. PhD preferred.

Minimum Experience:

Ten (10) years relevant work experience is required with aBachelor’s degree. Eight (8) years of relevant work experience is required with aMaster’s degree. Five (5) years of relevant work experience is required with aPhD.Have More in MemphisGet the best of big-city amenities mixedwith all the charm of a small-town feel when you live in Memphis.

From our world-renowned music scene to our eclectic mix of food andpeople. Memphis is a place our employees are proud to call home.

Our region enjoys a cost of living more than 20% lower than thenational average and our state places no personal income tax onwages. Plus, Memphians spend five hours less per year in theirdaily commute compared to the national average. Learn how you can”.” -CompensationIn recognition of certain U.S. state and municipalpay transparency laws, St. Jude is including a reasonable estimateof the compensation range for this role. This is an estimateoffered in good faith and a specific salary offer takes intoaccount factors that are considered in making compensationdecisions including but not limited to skill sets, experience andtraining, licensure and certifications, and other business andorganizational needs. It is not typical for an individual to behired at or near the top of the salary range and compensationdecisions are dependent on the facts and circumstances of eachcase. A reasonable estimate of the current salary range is $104,000- $186,160 per year for the role of Cryo-Electron Microscopy SeniorScientist in Blue Sky Protein Kinases.

Explore our exceptional !NoSearch FirmsSt. Jude Children’s Research Hospital does not acceptunsolicited assistance from search firms for employmentopportunities. Please do not call or email. All resumes submittedby search firms to any employee or other representative at St. Judevia email, the internet or in any form and/or method without avalid written search agreement in place and approved by HR willresult in no fee being paid in the event the candidate is hired bySt. Jude.