Lead Researcher in Blue Sky Protein Kinases Position Available In Shelby, Tennessee

Job Description:

We are looking for an experienced biochemist to participate inour exciting studies of the human protein kinome.

As part of thelargest BlueSky initiative, “Seeing the Invisible in ProteinKinases” at St. Jude Children’s Research Hospital, we are expandingour structural studies of the human kinome to identify novelkinase-binding ligands. We are looking for an experiencedbiochemist to aid in these efforts.

The candidate will work in afast-paced research environment, studying protein kinases bygenerating and characterizing high quality proteins, assessingbinding affinity and kinetics using various biophysical techniquesin collaboration with Nuclear Magnetic Resonance (NMR)spectroscopists and computational biologists. This role willinvolve working closely as part of a multidisciplinary teamincluding computational chemists, structural biologists,biochemists, and other biophysical specialists. The unparalleledNMR center at St. Jude includes three 600 MHz, two 700 MHz, six 800MHz, 850 MHz, and the first 1.1 GHz in theworld.

Responsibilities:

Perform tasks related to the research project independently,but within specific guidelines and subject to regular review bysupervisor or other research staff. Perform basic molecular biology techniques like polymerasechain reaction (PCR), vector construction, plasmid purification andsequencing analysis. Responsible for expression, purification, modification, andcharacterization of recombinant proteins from a variety of hostcells. Design and implement purification schemes and proteinanalytics, present results, and interpretation. Lead troubleshooting efforts for protein expression andpurification processes. Work effectively in a collaborative team environment whereresults are achieved through influence, cooperation, and theincorporation of multiple points of view.

Ideal Candidate:

Proven expertise in advanced molecular cloning techniques. Experience generating chimeric recombinant sequences,site-directed mutagenesis, random mutagenesis, codon optimization,and/or gene synthesis is highly desired. Strong background in protein biochemistry, protein purification(using affinity and traditional chromatographic techniques), andbiophysical characterization. Hands-on experience with instrumentation essential for proteinpurification and characterization, especially BioRad/AKTA FPLC, SDSPAGE and Western Blot. Work independently to perform protein analysis including (butnot limited to) CE SDS, ITC, SPR, U/HPLC, DSF, and cell basedfunctional assays. Excellent oral, presentation and written communication skillsrequired.

Minimum Education and/or

Training:

Bachelor’s degree in arelevant scientific area is required.

Minimum Experience:

5+ years ofresearch experience is required with a Bachelor’s degree. 3+ years of experience is required with a Master’s degree. No prior experience required with a PhD. Strong knowledge and experience in understanding andapplication of underlying principles related to techniques beingperformed. Experience in R D methods and processes in lab setting, andexecution of research projects for the laboratory preferred. Proven performance in earlier role/comparable role.

St. JudeChildren’s Research Hospital offers a remarkable opportunity toperform cutting edge research with outstanding institutionalsupport and exceptional shared core facilities in an environment ofopen collaboration, educational advancement, and trainingopportunities. St. Jude is consistently ranked on FortuneMagazine’s and Glassdoor’s “100 Best Places to Work” for companieswith 1,000 or more employees. We are located in the heart ofMemphis, Tennessee, a vibrant and friendly city at the historicAmerican crossroads of music, trade, food, and culture. Mildwinters and beautiful green outdoor spaces are part of the reasonswhy Forbes recently named Memphis the 4th happiest city in which towork. Cost of living and high quality of life are otherattractions: Money magazine listed Memphis as one of the leastexpensive cities in which to raise a child. St. Jude facilities andsalaries are highly competitive.

CompensationIn recognition ofcertain U.S. state and municipal pay transparency laws, St. Jude isincluding a reasonable estimate of the compensation range for thisrole. This is an estimate offered in good faith and a specificsalary offer takes into account factors that are considered inmaking compensation decisions including but not limited to skillsets, experience and training, licensure and certifications, andother business and organizational needs. It is not typical for anindividual to be hired at or near the top of the salary range andcompensation decisions are dependent on the facts and circumstancesof each case. A reasonable estimate of the current salary range is$60,320 – $105,040 per year for the role of Lead Researcher in BlueSky Protein Kinases.

Explore our exceptional !Diversity, Equity andInclusionSt. Jude Children’s Research Hospital has a diverse,global patient population and workforce, built on the principles ofdiversity, equity and inclusion. Our founder Danny Thomasenvisioned a hospital that would treat children of theworld-regardless of race, religion or a family’s ability to pay..Today, we continue the mission to advance cures and means ofprevention for pediatric catastrophic diseases through research andtreatment. As we accelerate this progress globally, we believe ourlegacy of diversity, equity and inclusion is foundational tosuccess. With the commitment of leaders at all levels of theorganization, we strive to ensure the St. Jude culture, leadershipapproaches and talent processes are equitable and culturallyresponsive. View our to learn about the hospital’s roots indiversity, equity and inclusion, where we are today and ouraspirations for an even better future.

No Search FirmsSt. JudeChildren’s Research Hospital does not accept unsolicited assistancefrom search firms for employment opportunities. Please do not callor email. All resumes submitted by search firms to any employee orother representative at St. Jude via email, the internet or in anyform and/or method without a valid written search agreement inplace and approved by HR will result in no fee being paid in theevent the candidate is hired by St. Jude. #J-18808-Ljbffr