Cell Engineering / Molecular Biology Scientist in Protein Technologies Center Position Available In Shelby, Tennessee

Cell Engineering / Molecular Biology Scientist in Protein Technologies Center St. Jude Children’s Research Hospital – 4.4 Memphis, TN Job Details Full-time $86,320 – $154,960 a year 2 hours ago Benefits Paid time off Qualifications Doctoral degree Mid-level 3 years Master’s degree Bachelor’s degree Doctor of Philosophy Molecular biology Full Job Description Protein Technologies Center (PTC) within the Department of Structural biology is seeking a scientist with experience in CRISPR/Cas9 genome-editing strategy to systematically tag endogenous proteins with fluorescent and affinity tags for structural, functional, and drug discovery studies; also, for investigating protein interactions and mapping protein localization. Better at Work. Better at Life. When you work at St. Jude, you’ll join a highly collaborative work culture that inspires you every day to be your best. With opportunities for learning and growth, you can shape a career path that is right for you while also enjoying all the benefits and stability of working for a world-class institution. This includes work-life balance with generous paid time-off and on-campus conveniences that make life a little easier. Join us and you’ll quickly see why St. Jude is consistently ranked by our employees on Glassdoor as a “Best Place to Work.” The PTC is a multidisciplinary team with capabilities in protein engineering, cloning, expression, purification, biophysical analysis of proteins and structure determination. Cryo Electron Microscopy has enabled us to visualize molecular and atomistic details inside the cell. Manipulation of cell using molecular engineering tools will help to understand the complex nature of cellular processes at molecular level. This is a bench-based scientist role where the scientist will edit the cell lines and confirm if the editing was successful and if genomic stability is maintained during the clonal line-generation process. The Scientist will be providing pivotal support to several structural cell biology projects both strategically and tactically. The World’s Brightest Minds Always Innovate At St. Jude Children’s Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That’s why we provide world-class facilities. State-of-the-art technologies. Extraordinary support. And a collaborative, bench-to-bedside environment where you can see, firsthand, how your science translates into survival for the children we serve. Quite simply, at St. Jude, we encourage you to dream big and stop at nothing when it comes to finding cures and saving children.

Responsibilities:

Engineer cell lines using CRISPR gene editing technology for generating gene knockouts and knock-ins (SNVs, gene tags, etc.) and confirm the edits. Generate stable cell lines using Non-viral and viral-based delivery systems. Implement advanced technologies to develop stable target-over expressed cell lines, pools, clones and NGS library preparation. Proven track record in molecular cloning (In-Fusion or LIC or PIPE or Gateway or FX; overlap extension PCR/Gibson assembly/BioBrick/recombineering), handling large and/or complex DNA constructs, including combinatorial libraries; site-directed and/or saturation mutagenesis. Minimum Education and/or

Training:

Bachelor’s degree in relevant scientific area is required.

Minimum Experience:

Eight (8) years of relevant work experience is required with a Bachelor’s degree. Six (6) years of relevant work experience is required with a Master’s degree. Three (3) years of relevant work experience is required with a PhD.

Preferred Experience:

PhD (with at least 5 years of postdoctoral experience) in molecular biology or synthetic biology, or any related discipline. Have More in Memphis Get the best of big-city amenities mixed with all the charm of a small-town feel when you live in Memphis. From our world-renowned music scene to our eclectic mix of food and people. Memphis is a place our employees are proud to call home. Our region enjoys a cost of living more than 20% lower than the national average and our state places no personal income tax on wages. Plus, Memphians spend five hours less per year in their daily commute compared to the national average. Learn how you can “Have More in Memphis.” Compensation In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $86,320 – $154,960 per year for the role of Cell Engineering / Molecular Biology Scientist in Protein Technologies Center. Explore our exceptional benefits! St. Jude is an Equal Opportunity Employer No Search Firms St. Jude Children’s Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.